Transfer Into Balloon-Injured Rat Carotid Arteries
نویسندگان
چکیده
We studied the ability of adenoviral vectors to achieve gene transfer into injured arteries. A recombinant adenoviral vector expressing a nuclear-targeted f-galactosidase gene was constructed and infused into balloon-injured rat carotid arteries. Three days after gene transfer, recombinant gene expression was assessed quantitatively by (1) measuring 13-galactosidase antigen and activity in tissue extracts and (2) histochemical staining and counting of cells expressing ,B-galactosidase. Exposure of injured carotid arteries to increasing concentrations of the vector (10' to 1010 plaque-forming units per milliliter) resulted in a dose-responsive increase in f-galactosidase expression, with peak expression of approximately 43 mU
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